Freshers Jobs MSc Biotech – Jubilant Biosys Trainee Research Associate
Freshers Jobs MSc Biotech – Jubilant Biosys Trainee Research Associate. Freshers MSc Biochemistry/ Biotechnology/ Molecular Biology/ Biophysics Apply. Jubilant Biosys Trainee Job – Research Associate In Structural Biology Dept. Interested and eligible applicants can check out all of the details on the same below:
Jubilant Biosys is hiring for Trainee Research Associate for Structural Biology Department.
DATE POSTED: 04/09/23
LOCATION: Bangalore, Karnataka
COMPANY: Jubilant Biosys Limited
FUNCTION: Research & Development
Job Responsibilities:
- Cell culture, Stable cell line generation in mammalian cells and confirmation with western blotting, ELISA etc.
- Recombinant DNA technology (Isolation of DNA, Transformation, Selection, Recombination, Restriction digestion)
- Cloning of genes, expression and purification of proteins
- Using E. coli and insect cell expression systems for the expression of recombinant protein targets.
- Purifying the target protein using various chromatographic techniques
- Characterization of the expressed recombinant proteins
- Setting up crystallization screens for various conditions and temperatures
Educational Qualifications:
Fresher in M.Sc (Biochemistry/ Biotechnology/ Molecular Biology/ Biophysics).
Desirable Skills:
- Good scientific knowledge about the basics on production of proteins for enzymatic assay and structural work.
- Flexibility and the ability to work in fast paced and team oriented environment.
Here are five possible interview questions along with their answers for the Trainee Research Associate position in the Structural Biology Department at Jubilant Biosys:
1. Can you explain the steps involved in generating a stable cell line in mammalian cells?
Answer: Generating a stable cell line involves transfecting mammalian cells with a plasmid containing the gene of interest, followed by a selection process using antibiotics or other selection markers. This ensures that only cells containing the gene of interest survive and replicate.
2. What is the significance of recombinant DNA technology in molecular biology, and how have you applied it in your previous studies or projects?
Answer: Recombinant DNA technology allows us to manipulate and combine genes from different sources, enabling the expression of specific proteins for various purposes. I have applied it in my previous projects to clone and express genes of interest for protein purification and functional studies.
3. Describe your experience with protein expression using E. coli and insect cell expression systems. What are the key differences between these systems?
Answer: I have experience with both systems. E. coli is commonly used for easy and cost-effective protein expression, while insect cells are preferred for post-translational modifications. The key difference is that insect cells can perform complex modifications like glycosylation, which E. coli cannot.
4. Can you name and briefly explain some chromatographic techniques you’ve used for purifying target proteins?
Answer: I have utilized techniques such as affinity chromatography, ion-exchange chromatography, and size-exclusion chromatography for protein purification. Affinity chromatography uses specific interactions with ligands, ion-exchange separates based on charge, and size-exclusion separates by size.
5. What steps are involved in setting up crystallization screens for protein crystals, and why is this important in structural biology?
Answer: Setting up crystallization screens involves mixing protein samples with various precipitants and conditions to encourage crystal growth. This is crucial in structural biology because obtaining well-diffracting protein crystals is the first step in determining a protein’s 3D structure via X-ray crystallography or cryo-EM.
These answers provide a solid foundation for discussing the candidate’s qualifications and experience in structural biology.
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